Title: Consistent and Heritable Alterations of DNA Methylation are Induced by Tissue Culture in Maize
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1 Title: Consistent and Heritable Alterations of DNA Methylation are Induced by Tissue Culture in Maize Authors: Scott C. Stelpflug, Steven R. Eichten, Peter J. Hermanson, Nathan M. Springer, Shawn M. Kaeppler Author Affiliations: Department of Agronomy, College of Agricultural and Life Sciences, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706 Department of Plant Biology, University of Minnesota, 1445 Gortner Avenue, St. Paul, MN 55108
منابع مشابه
Consistent and heritable alterations of DNA methylation are induced by tissue culture in maize.
Plants regenerated from tissue culture and their progenies are expected to be identical clones, but often display heritable molecular and phenotypic variation. We characterized DNA methylation patterns in callus, primary regenerants, and regenerant-derived progenies of maize using immunoprecipitation of methylated DNA (meDIP) to assess the genome-wide frequency, pattern, and heritability of DNA...
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Transposable elements (TEs) are capable of inducing heritable de novo genetic variation. The sequences capable of reactivation, and environmental factors that induce mobilization, remain poorly defined even in well-studied genomes such as maize. We treated maize tissue culture with the demethylating agent 5-aza-2-deoxcytidine and examined long-term tissue culture lines to discover silenced TEs ...
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Background Genomic imprinting is a heritable and developmentally essential phenomenon by which gene expression occurs in an allele-specific manner1. While the imprinted alleles are primarily silenced by DNA methylation, it remains largely unknown how methylation is targeted to imprinting control region (ICR), also called differentially methylated region (DMR), and maintained. Here we show that ...
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تاریخ انتشار 2014